Plasmonic bio/chemical sensing based on optical fibers combined with molecularly imprinted nanoparticles (nanoMIPs), which are polymeric receptors prepared by a template assisted synthesis, has been demonstrated as a powerful method to attain ultra-low detection limits, particularly when exploiting soft nanoMIPs, that are known to deform upon analyte binding [1]. This work, presents the development of a surface plasmon resonance (SPR) sensor in silica light-diffusing fibers (LDFs) functionalized with a specific nanoMIP receptor, entailed for the recognition of the protein human serum transferrin (HTR). Despite their great versatility, to date LDF-SPR were just reported functionalized with antibodies. Here, the innovative combination of a SPR-LDF platform and nanoMIPs led to the development of a sensor with an ultra-low limit of detection (LOD), equal to about 4 fM, and selective for its target analyte HTR. Worth to note, the SPR-LDF-nanoMIP sensor was mounted within a specially designed 3D-printed holder yielding to a measurement cell suitable for a rapid and reliable setup, easy for the scaling up of the measurements.
SENSING OF PROTEINS THROUGH A PLASMONIC BIOSENSOR BASED ON LIGHT-DIFFUSING FIBERS FUNCTIONALIZED WITH MOLECULARLY IMPRINTED NANOPARTICLES
M. Seggio
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2022-01-01
Abstract
Plasmonic bio/chemical sensing based on optical fibers combined with molecularly imprinted nanoparticles (nanoMIPs), which are polymeric receptors prepared by a template assisted synthesis, has been demonstrated as a powerful method to attain ultra-low detection limits, particularly when exploiting soft nanoMIPs, that are known to deform upon analyte binding [1]. This work, presents the development of a surface plasmon resonance (SPR) sensor in silica light-diffusing fibers (LDFs) functionalized with a specific nanoMIP receptor, entailed for the recognition of the protein human serum transferrin (HTR). Despite their great versatility, to date LDF-SPR were just reported functionalized with antibodies. Here, the innovative combination of a SPR-LDF platform and nanoMIPs led to the development of a sensor with an ultra-low limit of detection (LOD), equal to about 4 fM, and selective for its target analyte HTR. Worth to note, the SPR-LDF-nanoMIP sensor was mounted within a specially designed 3D-printed holder yielding to a measurement cell suitable for a rapid and reliable setup, easy for the scaling up of the measurements.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
